Detection of four foodborne pathogens based on magnetic separation multiplex PCR and capillary electrophoresis

Foodborne pathogen contamination is a major safety issue for many foods and is causing concern worldwide. In this study, a detection system based on magnetic separation, multiplex PCR (MPCR) and capillary electrophoresis (CE) technologies was developed for the simultaneous detection of four foodborne pathogens. Magnetic separation technology is used to rapidly capture pathogenic bacteria in food samples, and then a combination of MPCR and CE can be used to greatly increase detection sensitivity. The detection limit for bacterial DNA reached 10(-5)-10(-7 )ng mu L-1 and in the analysis of mocked food samples, the assay showed good sensitivity for bacterial detection ranging from 10(1) to 10(5) CFU mL(-1) with excellent specificity. Compared to similar detection methodologies, this technique avoids the need for time-consuming enrichment cultures, is more sensitive, and can be used to assay simultaneously four foodborne pathogens.

Automated summary

A new detection system using magnetic separation, MPCR, and CE technologies was developed to simultaneously detect four foodborne pathogens. The combination of magnetic separation, MPCR, and CE greatly increased detection sensitivity, with a detection limit for bacterial DNA of 10(-5)-10(-7)ng mu L-1. This technique showed good sensitivity and specificity in detecting bacteria in food samples, without the need for time-consuming enrichment cultures.