期刊
BIOTECHNOLOGY FOR BIOFUELS
卷 14, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13068-021-02043-4
关键词
Trichoderma reesei; Hypocreajecorina; Filamentous fungi; Industry strains; Cellulases; Degeneration; Chromatin
资金
- Christian Doppler Research Association
- Austrian Federal Ministry for Digital and Economic Affairs
- National Foundation for Research, Technology and Development
- Novozymes
The study reveals that spontaneous degeneration of Trichoderma reesei strains during large-scale cultivation can lead to a loss of cellulase formation. The chromatin condensation in relevant promoter regions, related to the production capacity of the strain, plays a role in the occurrence of degenerated strain population.
Background Even if the loss of production capacity of a microorganism is said to be a serious problem in various biotechnology industries, reports in literature are rather rare. Strains of the genera Trichoderma reesei are used for large-scale production of cellulases, which are needed in food and feed, textile, paper industries and biofuel production. Results Here, we describe the phenomenon of spontaneous degeneration of T. reesei strains during large-scale cultivation. The phenotype of the degenerated population is characterized most importantly by a loss of any cellulase formation. Interestingly, promoter regions of relevant genes had a more compact chromatin in the (cel -) strains compared to productive strains. For a systematic investigation of the phenomenon a protocol for artificially induced and lab-scaled strain degeneration was developed. This workflow allows to determine the degeneration rate and thus, to compare the occurrence of a degenerated population in differently productive strains on the one hand, and to monitor the success of any strategies to prevent or decrease the degeneration on the other hand. While highly productive strains have higher degeneration rates compared to moderate producers, the degeneration can hardly be triggered in moderate producers. The observed (cel -) phenotype is not caused by a mutation in the gene encoding the essential transactivator Xyr1. The development of a non-producing population is also not triggered by any compounds released by either producing or non-producing cells. Conclusions The extent of the occurrence of a degenerated strain population relates to the production capacity of the strain and goes along with chromatin condensation in relevant promoter regions.
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