期刊
BIOTECHNOLOGY FOR BIOFUELS
卷 14, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13068-021-02051-4
关键词
Clostridium carboxidivorans; Citrulline; Syngas fermentation; Alcohol; acid ratio
资金
- National Key Research and Development Program of China [2021YFC2100900]
- Science and Technology Project of Xinjiang Production and Construction Corps [2019AB009]
- National Natural Science Foundation of China [30271470, 21778024]
- National First-Class Discipline Program of Light Industry Technology and Engineering [LITE2018-06]
- Program of Introducing Talents of Discipline to Universities [111-2-06]
Exogenous addition of citrulline can promote the growth of Clostridium carboxidivorans P7 and increase intracellular ATP levels, which is significant for enhancing the production of biofuels.
Background Clostridium carboxidivorans P7 is capable of producing ethanol and butanol from inexpensive and non-food feedstock, such as syngas. Achieving improved ethanol and butanol production in the strain for industrial application depends on the energetics and biomass, especially ATP availability. Results This study found that exogenous addition of citrulline promoted accumulation of ATP, increased specific growth rate, and reduced the doubling time of C. carboxidivorans P7. In heterotrophic fermentation experiments, the addition of citrulline increased intracellular ATP by 3.39-fold, significantly enhancing the production of total alcohol (ethanol + butanol) by 20%. Moreover, in the syngas fermentation experiments, the addition of citrulline improved the level of intracellular ATP and the biomass by 80.5% and 31.6%, respectively, resulting in an 18.6% and 60.3% increase in ethanol and the alcohol/acid production ratio, respectively. Conclusions This is the first report that citrulline could promote the growth of C. carboxidivorans P7 and increase the level of intracellular ATP, which is of great significance for the use of C. carboxidivorans P7 to synthesize biofuels.
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