期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 119, 期 2, 页码 470-481出版社
WILEY
DOI: 10.1002/bit.27984
关键词
cutinase; enzyme kinetics; PET hydrolase; structure-function analysis; substrate specificity
资金
- Novo Nordisk Fonden [NNFSA170028392]
- Knut och Alice Wallenbergs Stiftelse
- Osterreichische Forschungsforderungsgesellschaft
This study rigorously characterized two almost identical cutinases and demonstrated that even minor sequence differences in cutinases can drastically affect their substrate binding, substrate specificity, and catalytic efficiency.
Cutinases can play a significant role in a biotechnology-based circular economy. However, relatively little is known about the structure-function relationship of these enzymes, knowledge that is vital to advance optimized, engineered enzyme candidates. Here, two almost identical cutinases from Thermobifida cellulosilytica DSM44535 (Thc_Cut1 and Thc_Cut2) with only 18 amino acids difference were used for a rigorous biochemical characterization of their ability to hydrolyze poly(ethylene terephthalate) (PET), PET-model substrates, and cutin-model substrates. Kinetic parameters were compared with detailed in silico docking studies of enzyme-ligand interactions. The two enzymes interacted with, and hydrolyzed PET differently, with Thc_Cut1 generating smaller PET-degradation products. Thc_Cut1 also showed higher catalytic efficiency on long-chain aliphatic substrates, an effect likely caused by small changes in the binding architecture. Thc_Cut2, in contrast, showed improved binding and catalytic efficiency when approaching the glass transition temperature of PET, an effect likely caused by longer amino acid residues in one area at the enzyme's surface. Finally, the position of the single residue Q93 close to the active site, rotated out in Thc_Cut2, influenced the ligand position of a trimeric PET-model substrate. In conclusion, we illustrate that even minor sequence differences in cutinases can affect their substrate binding, substrate specificity, and catalytic efficiency drastically.
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