期刊
BIOTECHNOLOGY ADVANCES
卷 54, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2021.107866
关键词
Genomics; Synthetic biology; Microbial chassis; Biosynthetic gene cluster; Promoter; Metabolic engineering; Compartmentalization
资金
- National Key R&D Program of China [2019YFA0905700]
- National Natural Science Foundation of China [31800047, 31970071]
- Major Basic Research of Shandong Provincial Natural Science Foundation [ZR2019ZD19]
- Young Scholars Program of Shandong University
Natural products from fungi are important for therapeutic agent development, but many fungal biosynthetic gene clusters (BGCs) are not expressed under laboratory conditions, necessitating the development of new expression platforms. Therefore, increasing heterologous expression of BGCs is a current research focus.
Natural products from fungi represent an important source of biologically active metabolites notably for therapeutic agent development. Genome sequencing revealed that the number of biosynthetic gene clusters (BGCs) in fungi is much larger than expected. Unfortunately, most of them are silent or barely expressed under laboratory culture conditions. Moreover, many fungi in nature are uncultivable or cannot be genetically manipulated, restricting the extraction and identification of bioactive metabolites from these species. Rapid exploration of the tremendous number of cryptic fungal BGCs necessitates the development of heterologous expression platforms, which will facilitate the efficient production of natural products in fungal cell factories. Host selection, BGC assembly methods, promoters used for heterologous gene expression, metabolic engineering strategies and compartmentalization of biosynthetic pathways are key aspects for consideration to develop such a microbial platform. In the present review, we summarize current progress on the above challenges to promote research effort in the relevant fields.
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