期刊
BIOSENSORS & BIOELECTRONICS
卷 192, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113504
关键词
Urinary exosome; miRNA; Hydrogel microparticle; Diagnostics; Prostate cancer
类别
资金
- KIST Institutional Program [2E30965]
- KU-KIST Graduate School of Converging Science and Technology Program
- Bio & Medical Technology Development Program [NRF-2020M3A9D8039920]
- National Research Foundation of Korea (NRF) - Ministry of Science ICT [NRF-2016R1A5A1010148]
- Rural Development Administration of the Republic of Korea [PJ016004]
- Nanoconnect Program [NRF-2021M3H4A4079294]
Urinary exosomal miRNAs are potential biomarkers, but limited by low concentration and small volume. Hydrogel-based HCR technique shows high amplification and enhanced detection limits for miRNA in urine samples, with potential for clinical diagnosis applications in differentiating prostate cancer patients from healthy controls.
Although urinary exosomal microRNAs (miRNAs) have recently emerged as potential biomarkers, clinical applications are still limited due to their low concentration in small volumes of clinical samples. Therefore, the development of a non-invasive, specific diagnostic tool, along with profiling exosomal miRNA markers from urine, remains a significant challenge. Here, we present hydrogel-based hybridization chain reaction (HCR) for multiplex signal amplification to detect urinary exosomal miRNAs from human clinical samples. We succeeded in identifying small amounts (similar to amol) of exosomal miRNAs from 600 mu L of urine with up to similar to 35-fold amplification and enhanced detection limits by over an order of magnitude for two miRNA biomarker candidates, hsa-miR6090 and hsa-miR-3665. Furthermore, we proposed ratiometric analysis without requiring normalization to a reference miRNA and validated the clinical diagnostic potential toward differentiating prostate cancer patients from healthy controls. Our hydrogel-based HCR could serve as a new diagnostic platform for a non-invasive liquid biopsy before burdensome tissue biopsy of various diseases, including prostate cancer screening, complementing the PSA test.
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