期刊
BIOSENSORS & BIOELECTRONICS
卷 189, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113373
关键词
Aflatoxin B1; Photoelectrochemical immunosensor; Z-scheme heterojunction; g-C3N4/Au/WO3
类别
资金
- National Natural Science Foundation of China [51872140, 51972173]
- program for Science and Technology Innovative Research Team in Universities of Jiangsu Province, China
A label-free photoelectrochemical immunosensor using a Z-scheme heterojunction of gold nanoparticles loaded on graphitic carbon nitride sheet and tungsten trioxide sphere composite was fabricated for sensitive detection of Aflatoxin B1. The immunosensor displayed a linear concentration range from 1.0 pg mL(-1) to 100 ng mL(-1) with a low detection limit of 0.33 pg mL(-1) for AFB1. Good reproducibility, stability, and specificity provide a solid foundation for practical application.
Aflatoxin B1 (AFB1) is the most toxic mycotoxin, is widely found in foods and animal feeds, and can pose a serious threat to our lives. A label-free photoelectrochemical (PEC) immunosensor was fabricated for the sensitive detection of AFB1. A Z-scheme heterojunction of gold nanoparticles (Au NPs) loaded on graphitic carbon nitride sheet and tungsten trioxide sphere composite (g-C3N4/Au/WO3) acted as the highly sensitive platform. The g-C3N4/Au/WO3 is capable, not only of immobilizing antibodies via Au NPs, but also enhancing the separation of electron-hole pairs due to its good energy band matching efficiency. The mechanism of photo-generated electron/hole transfer on g-C3N4/Au/WO3 was explored using scavengers to eliminate active components. On this basis, an electron transfer pathway for the immunosensor was deduced. The PEC immunosensor displayed a linear concentration range from 1.0 pg mL(-1) to 100 ng mL(-1) and a low detection limit of 0.33 pg mL(-1) (S/N = 3) for AFB1. Good reproducibility, stability, and specificity provide a solid foundation for the practical application of this immunosensor.
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