期刊
BIOSENSORS & BIOELECTRONICS
卷 189, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113375
关键词
MoS2; Biosensor; CNDs; COVID-19; Listeria
类别
资金
- Comunidad de Madrid [S2013/MIT-3029, S2013/MIT-3007, 2017-T1/BIO-5435, MAD2D-CM S2013/MIT-3007]
- Ministerio de Economia, Industria y Competitividad [CTQ 2015-71955-REDT, CTQ 2014-53334-C2-1-R, MAT 2015-71879-P]
- European Research Council [ERC-PoC-842606]
- MINECO [CTQ 2017-86060-P]
- 'Severo Ochoa' Programme for Centres of Excellence in RD (MINECO) [SEV-2016-0686]
- UAM
- Banco Santander
- Fundacion IMDEA [10.01.03.02.41]
This research focuses on the development of DNA biosensors for selective and sensitive detection of pathogens by combining MoS2 and a` la carte functionalized CNDs. Experimental results show successful detection of Listeria monocytogenes and SARS-CoV-2, with potential applications for other pathogens that have their DNA sequenced.
This work focuses on the combination of molybdenum disulfide (MoS2) and a` la carte functionalized carbon nanodots (CNDs) for the development of DNA biosensors for selective and sensitive detection of pathogens. MoS2 flakes prepared through liquid-phase exfoliation, serves as platform for thiolated DNA probe immobilization, while thionine functionalized carbon nanodots (Thi-CNDs) are used as electrochemical indicator of the hybridization event. Spectroscopic and electrochemical studies confirmed the interaction of Thi-CNDs with DNA. As an illustration of the pathogen biosensor functioning, DNA sequences from InIA gen of Listeria monocytogenes bacteria and open reading frame sequence (ORF1ab) of SARS-CoV-2 virus were detected and quantified with a detection limit of 67.0 fM and 1.01 pM, respectively. Given the paradigmatic selectivity of the DNA hybridization, this approach allows pathogen detection in the presence of other pathogens, demonstrated by the detection of Listeria monocytogenes in presence of Escherichia coli. We note that this design is in principle amenable to any pathogen for which the DNA has been sequenced, including other viruses and bacteria. As example of the application of the method in real samples it has been used to directly detect Listeria monocytogenes in cultures without any DNA Polymerase Chain Reaction (PCR) amplification process.
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