期刊
BIOSENSORS & BIOELECTRONICS
卷 194, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113615
关键词
Small extracellular vesicles; Multiplexed droplet digital PCR; Machine learning; Breast cancer
类别
资金
- Research Grants Council of Hong Kong under General Research Fund [16205619]
- National Natural Science Foundation of China [81902165, 81672076]
- Natural Science Foundation of Guangdong Province [2019A1515011077]
- Basic and Applied Basic Research Programs of Guangdong Province [2019B1515120074]
- Guangdong Medical Science and Technology Research Fund Project [A2019458]
- President Foundation of Nanfang Hospital, Southern Medical University [2018C004]
The study introduced a 4-plex droplet digital PCR technology coupled with machine learning algorithms for simultaneous detection of four sEV-derived mRNAs to improve breast cancer diagnosis. The results showed that ML-assisted analysis exhibited higher diagnostic performance and improved efficiency in breast cancer diagnosis. Multiple sEV-derived mRNAs analysis coupled with ML not only provides the best combination of markers for breast cancer diagnosis, but also significantly improves the diagnostic efficiency of breast cancer.
Breast cancer has become the leading cause of global cancer incidence and a serious threat to women's health. Accurate diagnosis and early treatment are of great importance to prognosis. Although clinically used diagnostic approaches can be used for cancer screening, accurate diagnosis of breast cancer is still a critical unmet need. Here, we report a 4-plex droplet digital PCR technology for simultaneous detection of four small extracellular vesicle (sEV)-derived mRNAs (PGR, ESR1, ERBB2 and GAPDH) in combination with machine learning (ML) algorithms to improve breast cancer diagnosis. We evaluate the diagnsotic results with and without the assistance of the ML models. The results indicate that ML-assisted analysis exhibits higher diagnostic performance even using a single marker for breast cancer diagnosis, and demonstrate improved diagnostic performance under the best combination of biomarkers and suitable ML diagnostic model. Therefore, multiple sEV-derived mRNAs analysis coupled with ML not only provides the best combination of markers for breast cancer diagnosis, but also significantly improves the diagnostic efficiency of breast cancer.
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