期刊
BIOSENSORS & BIOELECTRONICS
卷 198, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113827
关键词
Intracellular ATP detection; 3xF30 tandem method; Fluorescent light-up aptamer; Heterobifunctional aptamers
类别
资金
- National Key Research and Development Project [2017YFA0504300]
- Science and Technology Program of Sichuan Province [2020YFS0069]
- Chengdu Science and Technology Bureau [2021-YF05-01681-SN]
- Fundamental Research Funds for the Central Universities [YJ201920]
A novel label-free fluorescent turn-on biosensor for monitoring ATP in living bacterias and mammalian cells was developed, utilizing heterobifunctional aptamers with high sensitivity. A tandem method combining Broc-ATPs with 3 x F30 three-way junction scaffold was established to construct an intracellular biosensor for sensitive and specific imaging of ATP dynamics in mammalian cells.
Visualizing the dynamics of ATP in living cells is key to understanding cellular energy metabolism and related diseases. However, the live-cell applications of current methods are still limited due to challenges in biological compatibility and sensitivity to pH. Herein, a novel label-free fluorescent turn-on biosensor for monitoring ATP in living bacterias and mammalian cells was developed. This biosensor (Broc-ATP) employed heterobifunctional aptamers to detect ATP with high sensitivity in vitro. In our system, a very useful tandem method was established by combining four Broc-ATPs with 3 x F30 three-way junction scaffold to construct an intracellular biosensor that achieves sufficient fluorescence to respond to intracellular ATP. This intracellular biosensor can be used for sensitive and specific dynamic imaging of ATP in mammalian cells. Hence, this genetically encoded biosensor provides a robust and efficient tool for the detection of intracellular ATP dynamics and 3 x F30 tandem method expands the application of heterobifunctional aptamers in mammalian cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据