期刊
BIOSENSORS & BIOELECTRONICS
卷 206, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2022.114085
关键词
Whole-cell biosensor; Synthetic biology; Genetic circuits; Quorum sensing; Degradation
类别
资金
- National Key Research and Development Program of China [2016YFA0204300]
- National Natural Science Foundation of China [22078096]
- Natural Science Foundation of Shanghai [18ZR1409900]
In this study, smart whole-cell biosensors were created by combining synthetic biology approaches with the bacterial quorum sensing mechanism. The biosensors demonstrated high sensitivity for phenolic compounds and the capability to degrade methyl parathion, an organophosphate pesticide. The design of these biosensors can be used to develop more efficient sensors for detecting and degrading environmental pollutants.
The environmental accumulation of organophosphates is a serious threat to public health. To detect these xenobiotics, a broad range of sensors has been developed in past decades. However, sensors with high sensitivity and a capability for degrading organophosphates are rare. In this study, smart whole-cell biosensors were created by combining synthetic biology approaches with the bacterial quorum sensing (QS) mechanism. The engineered whole-cell biosensor pUC57-QS-DSF-F42 L/E coli DH5 alpha can sense a wide array of phenolic compounds including phenol and p-nitrophenol (p-NP). By optimizing the genetic circuits, the phenol and p-NP detection limits reached 0.1 and 1 mu M, respectively. Importantly, by replacing the fluorescence-generated reporter sfGFP with MP-degrading enzyme PoOPHM2, the whole-cell biosensor pUC57-OPH-QS-DSF-F42 L/E coli DH5 alpha actively degraded 10 and 100 mu M methyl parathion (MP), a typical organophosphate pesticide, which was artificially added to the cell culture at different time points in five consecutive degrading experiments, demonstrating its MP sensing and degrading capabilities. The universal design of this new biosensor can be used to create more efficient biosensors to detect and degrade various pollutants in the environment for rapid testing and bioremediation.
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