4.8 Article

Quantitative detection of aflatoxin B1 using quantum dots-based immunoassay in a recyclable gravity-driven microfluidic chip

期刊

BIOSENSORS & BIOELECTRONICS
卷 190, 期 -, 页码 -

出版社

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113394

关键词

AflatoxinB1; Microfluidic chip; Quantum dot; Fluorescence immunoassay; Physical simulation

资金

  1. National Key Research and Development Program of China [2017YFC1600403]
  2. Guangdong Provincial Key Laboratory [2020B121201009]
  3. Guangdong Academy of Sciences Special Project of Implementing Innovation-Driven Development Capacity Building [2018GDASCX-0401]

向作者/读者索取更多资源

A novel microfluidic chip was developed for rapid and sensitive detection of AFB1, showing a wide analytical range and low detection limit, as well as good specificity and reusability. The chip design provides a reliable scheme for on-site detection of AFB1 and analysis of other small molecules.
To achieve rapid and sensitive detection of aflatoxin B1 (AFB1), we developed a polydimethylsiloxane gravitydriven cyclic microfluidic chip using the two-signal mode strategy. The structural design of the chip, together with the two-wavelength quantum dot ratio fluorescence, effectively eliminates the influence of environmental factors, improves the signal stability, and ensures that the final detection result positively correlates with the target concentration. Moreover, the theoretical analysis performed for the established physical model of the three-dimensional reaction interface inside the chip confirmed the improved reaction rate of immune adsorption in the microfluidic strategy. Overall, the method exhibited a wide analytic range (0.2-500 ng mL-1), low detection limit (0.06 ng mL-1), high specificity, good precision (coefficient of variation < 5%), excellent reusability (20 times, 89.1%) and satisfactory practical sample analysis capacity. Furthermore, the reusability and designability of this chip provide a reliable scheme for field detection of AFB1, analysis of other small molecules, and establishment of high-throughput detection systems under different conditions.

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