期刊
BIOSENSORS & BIOELECTRONICS
卷 197, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113758
关键词
ATP; Fe3O4@COF-Au NPs; Hybridization chain reaction; Electro-catalytic
类别
资金
- NNSF of China [21775124, 21974108]
- Fundamental Research Funds for the Central Univer-sities [XDJK2019B022]
- Chongqing Research Program of Basic Research and Frontier Technology [cstc2018jcyjA0797]
This study developed a signal-off electrochemical biosensor using Fe3O4@COF-Au NPs as a nanocarrier for sensitive detection of ATP. The Fe3O4@COF-Au NPs effectively confined Au NPs in uniform channels, providing a stable micro-environment for enhanced electro-catalytic performance. This approach not only enables sensitive ATP detection but also offers a versatile strategy for monitoring other biomolecules.
In this work, a signal-off electrochemical biosensor was established for sensitive detection of adenosine triphosphate (ATP) based on Fe3O4@covalent organic framework-immobilized gold nanoparticles (Fe3O4@COF-Au NPs) porous composite material as a nanocarrier. The proposed Fe3O4@COF-Au NPs could effectively confine Au NPs in the uniform channels of the Fe3O4@COF, which successfully avoided Au NPs aggregation to a certain extent and provided a comparatively independent and stable micro-environment via its hydrophobic porous nanochannels, thereby owning excellent electro-catalytic performance for the reduction of 4-nitrophenol. Moreover, the Fe3O4@COF-Au NPs nanomaterials were served as functional platform for immobilizing DNA substrate (SO), which was used to bind with the conversion product (S1) of the target ATP for subsequent branched hybridization chain reaction (b-HCR) to form dendritic DNA strands to hinder electron transfer between Fe3O4@COF-Au NPs and 4-nitrophenol, finally achieving sensitive detection of ATP with a wide linear range of 5 pM-50 mu M and a low detection limit of 1.6 pM. Such strategy provides a multifunctional immobilized platform for the sensitive detection of ATP and a versatile strategy for monitoring other biomolecules.
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