期刊
BIOSENSORS & BIOELECTRONICS
卷 197, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113773
关键词
Ag deposition; Immunosensor; Thyroid-stimulating hormone; Redox cycling; Diaphorase; Quinone
类别
资金
- National Research Foundation of Korea [2021R1A2C3012115, 2019R1A4A1028007, 2017M3A7B4041973]
- National Research Foundation of Korea [2021R1A2C3012115, 2017M3A7B4041973, 2019R1A4A1028007] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study presents a rapid silver deposition method using enzyme and quinone substrate without the need for a washing step, showing great potential for ultrasensitive clinical applications in detecting thyroid-stimulating hormone (TSH) with high sensitivity and promising results.
Enzyme-induced seedless Ag deposition is useful for selective Ag deposition and subsequent electrochemical Ag oxidation; however, a washing step is required after the deposition and before the electrochemical oxidation as the enzyme substrate can be oxidized during the electrochemical oxidation. Here, we report a fast Ag deposition method using a redox enzyme and quinone substrate that does not require a washing step. We found that the quinone substrate is reduced by a redox enzyme label, which is later oxidized to its original form via the reduction of Ag+ to Ag. Moreover, the quinone substrate is not electrochemically oxidized during the electrochemical Ag oxidation. We selected one diaphorase and 1,4-naphthoquinone from among seven redox enzymes (four diaphorases and three glucose-oxidizing enzymes) and six quinones, respectively. We applied this Ag deposition method for the detection of thyroid-stimulating hormone (TSH) over a dynamic range from 100 fg/mL to 100 ng/mL and found that TSH could be detected at concentrations as low as approximately 100 fg/mL in artificial serum. Therefore, the Ag deposition strategy developed in this study exhibits promising potential for ultrasensitive clinical applications.
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