期刊
BIOSENSORS & BIOELECTRONICS
卷 189, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113385
关键词
Surface plasmon resonance; Magnetite nanorods containing ordered; mesocages; Silver nanoclusters; PD-L1; Dual selective recognition
类别
资金
- National Natural Science Foundation of China [61875114and 62005156]
A highly specific surface plasmon resonance biosensor was designed for the detection of PD-L1 using magneto-optical nanocomplexes, which provided dual selective recognition of PD-L1 and showed good linear range for PD-L1 detection. The practical performance of this immunosensing platform was successfully verified by clinical samples, offering a new strategy for point-of-care detection of PD-L1.
Programmed death ligand 1 (PD-L1) is a typical immune checkpoint protein, whose up-regulation on the membrane of different tumor cells inhibits the immune response of T cells and leads to the escape of tumor cells. In this work, we designed a facile and highly specific surface plasmon resonance (SPR) biosensor to detect PD-L1 in human plasma based on magnetite nanorods containing ordered mesocages (MNOM) and silver nanoclusters (AgNCs). Magneto-optical nanocomplex MNOM@AgNCs with superior magneto-optical properties and high signal-to-noise ratio were fabricated to improve the detection sensitivity owing to the high specific surface area of MNOM and excellent localized SPR of AgNCs. The PD-L1 Antibody on the surface of gold chip and the PD-L1 aptamer on MNOM@AgNCs could realize dual selective recognition of PD-L1, providing the specificity of the sensor and reducing non-specific binding. The SPR sensor showed a good linear range of PD-L1 from 10 ng/mL to 300 ng/mL with the detection limit of 3.29 ng/mL. The practical performance of this immunosensing platform had been successfully verified by clinical samples which included healthy donors and cancer patients. Based on the analysis, the developed immunosensor provided a new strategy for point-of-care detection of PD-L1 and could be used as clinical companion diagnosis of PD-1/PD-L1 inhibitor therapy.
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