期刊
BIOSENSORS & BIOELECTRONICS
卷 195, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113673
关键词
Gas sensor; Native chemical ligation; Olfactory receptor-derived peptide; Single-walled carbon nanotube; Steglich esterification reaction; Trimethylamine
类别
资金
- National Natural Science Foundation of China [61871285, 21974094]
- Open Project Program of the State Key Laboratory of Proteomics [SKLP-O202004]
A high-performance TMA sensor was developed using a chemical connection method to effectively load ORPs onto SWCNTs, achieving detection of TMA at concentrations as low as 0.01 parts per trillion, three orders of magnitude lower than previously reported. The sensor demonstrated excellent sensitivity, selectivity, and stability for TMA detection, showing great potential for applications in environmental safety, food quality evaluation, and healthcare.
Trimethylamine (TMA) commonly exists in daily life and is harmful to human health, therefore the convenient and sensitive monitoring of TMA is highly desired. In this study, we developed a method to fabricate a high-performance TMA sensor by chemically conjugating olfactory receptor-derived peptides (ORPs) to single-walled carbon nanotubes (SWCNTs) on interdigital electrodes. First, the SWCNTs were modified with thioester by Steglich esterification reaction. Next, the ORPs with a cysteine residue at the N-terminus were connected to the thioester by native chemical ligation and modified to the surface of the SWCNTs. The chemical connection method enabled more effective loading of ORPs to the SWCNTs compared to the previously reported physical connection method. Using this approach, the ORPs-SWCNTs sensor for gaseous TMA was fabricated and enabled detection of TMA with a concentration as low as 0.01 parts per trillion, which was three orders of magnitude lower than the reported lowest detection limit up to date. Furthermore, we tested the performance of the ORPsensor with vaporized TMA and TMA generated from various spoiled food, and the sensor exhibited excellent sensitivity, selectivity, and stability for TMA detection. The results demonstrated the effectiveness of the proposed chemical connection method for the fabrication of ORP-sensor and the great potential of using these sensors for applications in environmental safety, food quality evaluation, and healthcare.
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