期刊
BIOSENSORS & BIOELECTRONICS
卷 194, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113569
关键词
Bioluminescence; Colorimetric; Paper sensor; Smartphone; Mercury
类别
资金
- NATO Science For Peace And Security Programme [985042]
- FEDKITO project - MIUR
- UK Research and Innovation Future Leaders Fellowship [MR/S018875/1]
- US Office of Naval Research Global grant [N62909-20-1-2036]
- FEDKITO project - Prima (Partnership for Research and Innovation in the Mediterranean Area)
- UKRI [MR/S018875/1] Funding Source: UKRI
This low-cost biosensor integrates three different biorecognition principles for orthogonal detection of mercury(II) on a three-leaf paper, providing a stable disposable device that can easily be inserted into a smartphone for mercury detection in water samples, with a detection limit reaching ppb levels.
Mercury contamination in the environment has reached alarming levels. Due to its persistence and bioaccumulation, mercury is one of the most widespread toxic heavy metals found in air, water and food. Thus, it is mandatory to monitor mercury and its compounds, and the availability of sensitive and rapid biosensors is highly valuable. We developed a low-cost biosensor for orthogonal detection of mercury(II) integrating three different biorecognition principles on a three-leaf paper: i) a mercury-specific bioluminescent Escherichia coli bioreporter strain expressing NanoLuc luciferase as reporter protein, ii) a purified beta-galactosidase (beta-gal) enzyme which is irreversibly inhibited by mercury and other metal ions, and iii) an Aliivibrio fischeri bioluminescent strain which is used to quantitatively assess sample toxicity and correct the analytical signal accordingly. Both sensory elements and substrates, Furimazine for the bioluminescent reporter strain and chlorophenol red beta-D-galactopyranoside for colorimetric detection of beta-gal, were integrated in the paper sensor to provide a stable all-in-one disposable cartridge which can be easily snapped into a smartphone with a clover-shaped 3D printed housing. This is the first integration of bioluminescence and colorimetric detection on a smartphone-paper sensor, providing a readout within 15 and 60 min for the colorimetric and bioluminescent detection respectively. The biosensor was applied to water samples spiked with different concentrations of mercury, interferents and toxic chemicals providing a limit of detection for Hg(II) at the ppb levels.
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