期刊
BIOSENSORS & BIOELECTRONICS
卷 194, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113594
关键词
Microvesicles; Exosomes; Exosome separation; Exosome detection; Microfluidic chip
类别
资金
- National Natural Science Foundation of China [82003274, 61774106]
- Science and Technology Commission of Shanghai Municipality [20JC1415000, 21S31906400]
- China Postdoctoral Science Foundation [2020M681333]
- Medical-Engineering Cross Foundation of Shanghai Jiao Tong University [YG2021QN58]
The ExoSD chip is a highly integrated platform for exosome separation and detection, capable of efficiently separating and detecting gastric cancer cell-derived exosomes with high recovery and purity.
Gastric cancer cell-derived exosomes as biomarkers have a very high application potential to the non-invasive detection of early-stage gastric cancer. However, the small size of exosomes (30-150 nm) results in huge challenges in separating and detecting them from complex media (e.g., plasma, urine, saliva, and cell culture supernatant). Here we proposed a highly integrated exosome separation and detection (ExoSD) chip to immunomagnetic separate exosomes from cell culture supernatant in a manner of continuous flow, and to immunofluorescence detect gastric cancer cell-derived exosomes with high sensitivity. The ExoSD chip has achieved a high exosome recovery (>80%) and purity (>83%) at the injection rate of 4.8 mL/h. Furthermore, experimental results based on clinical serum samples of patients with gastric cancer (stages I and II) show that the detection rate of the ExoSD chip is as high as 70%. The proposed ExoSD chip has been successfully demonstrated as a cutting-edge platform for exosomes separation and detection. It can be served as a versatile platform to extend to the applications of separation and detection of the other cell-derived exosomes or cells.
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