期刊
BIOSENSORS & BIOELECTRONICS
卷 195, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113649
关键词
COVID-19; Electrochemical biosensor; SARS-CoV-2 RNA; S gene; Orf1ab gene
类别
资金
- Korea government's National Research Foundation (NRF) [2020R1A5A1018052]
- Ministry of Trade, Industry and Energy [20008763]
The study introduces a novel nucleic acid amplification-free electrochemical biosensor for rapid and simultaneous detection of different genes of SARS-CoV-2, showing promising performance in clinical samples and potential for on-site, real-time diagnostic testing for COVID-19.
Considering the worldwide health crisis associated with highly contagious severe respiratory disease of COVID19 outbreak, the development of multiplexed, simple and rapid diagnostic platforms to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is in high demand. Here, a nucleic acid amplification-free electrochemical biosensor based on four-way junction (4-WJ) hybridization is presented for the detection of SARS-CoV-2. To form a 4-WJ structure, a Universal DNA-Hairpin (UDH) probe is hybridized with two adaptor strands and a SARS-CoV-2 RNA target. One of the adaptor strands is functionalized with a redox mediator that can be detected using an electrochemical biosensor. The biosensor could simultaneously detect 5.0 and 6.8 ag/mu L of S and Orf1ab genes, respectively, within 1 h. The biosensor was evaluated with 21 clinical samples (16 positive and 5 negative). The results revealed a satisfactory agreement with qRT-PCR. In conclusion, this biosensor has the potential to be used as an on-site, real-time diagnostic test for COVID-19.
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