期刊
BIOSENSORS & BIOELECTRONICS
卷 192, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113539
关键词
Crown monitoring; Caspase-3; H2O2; Imaging; FRET/SERS
类别
资金
- National Natural Science Foundation of China [21904068, 21906019, 81922041]
- Natural Science Foundation of Jiangsu Province [BK20201351]
- Science and Technology Department of Jiangsu Province [19KJB150014]
- Hong Kong Scholars Programme [XJ2017014]
- Introduction of Talent Research Start Fund of Nanjing Medical University [KY101RC20190007]
In this study, a new strategy for simultaneous detection of different substances on a single probe was developed. The fabricated AuNPL-crown nanoprobe allows for dual-channel and real-time tracking of the dynamic changes in caspase-3 and H2O2. This new platform provides high accuracy, sensitivity, convenience, and efficiency for the analysis of caspase-3 and H2O2 in the biological environment.
Caspase-3 and hydrogen peroxide (H2O2) are closely associated with numerous diseases, both of them are vital in different physiological and pathological conditions. They are closely related and also can act independently. The selective and accurate determination of caspase-3 and H2O2 simultaneously to determine their state of being in different situations is of great significance for further study of their molecular mechanisms and the elucidation of their biological functions. In our latest research, a AuNPL-crown nanoprobe was obtained by attaching (4-aminosulfonylphenyl) boronic acid (4-APBA) and peptide-FITC (NH2-Asp-Glu-Val-Asp (DEVD)-FITC) to gold nanoplates (AuNPLs). The fabricated AuNPL-crown nanoprobe was used for dual-channel and real-time tracking of the dynamic changes in caspase-3 and H2O2 based on fluorescence resonance energy transfer (FRET)/surfaceenhanced Raman spectroscopy (SERS) technology. The AuNPL-crown nanoprobe not only provides synergy but can also achieve noninterference, making the results more reliable and repeatable. This study simultaneously traced the dynamic changes of caspase-3 and H2O2 on a single probe, which provides a potential new platform for the analysis of caspase-3 and H2O2 in the biological environment with high accuracy, sensitivity, convenience, and efficiency. In summary, we develop a new strategy for the simultaneous detection of different substances on a single probe.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据