期刊
BIORESOURCE TECHNOLOGY
卷 341, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125830
关键词
ACC deaminase; Antioxidant enzymes; Ethylene; Lipid; Nitrogen deficit
资金
- National Post-doctoral Fellowship Scheme (NPDF) by Science and Engineering Research Board (SERB), Government of India [PDF/2018/000789]
The study demonstrated that expressing the ACC deaminase gene in Chlamydomonas led to improved tolerance to nitrogen deficiency and enhanced growth activity, as well as increased lipid and fatty acid content.
1-Aminocyclopropane-1-carboxylate (ACC) deaminase is a well-known bacterial producing enzyme that helps plants to overcome stress conditions by modulating ethylene biosynthesis. However, the functional role of ACC deaminase and ethylene in microalgae during stress remains to be explored. In this study, to investigate the role of ACC deaminase (acds) from Pseudomonas putida UW4 in enhancing the biomass and lipid content of Chlamydomonas under nitrogen deficit condition. The synthetic codon-optimized acds gene was cloned into vector pChlamy_4 and introduced into Chlamydomonas. Results indicated that Chlamydomonas-expressing acds lines showed significant tolerance to nitrogen-deficit by reducing the ethylene content. The biomass, chlorophyll content and photosynthetic activity of acds-expressing lines were significantly increased during nitrogen deficit condition. Moreover, the intracellular lipid and fatty acid content were much higher in acds-expressing lines than the wild-type. In terms of stress alleviation, the transgenic lines displayed increased antioxidant enzymes, reduced ROS and lipid peroxidation levels.
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