期刊
BIORESOURCE TECHNOLOGY
卷 337, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125436
关键词
P-coumaric acid; In situ product recovery; Engineered Saccharomyces cerevisiae; 4-vinylphenol; Biphasic fermentation
资金
- Agence Nationale de la Recherche (ANR) [ANR-17-CE07-0046]
- Region Grand Est (France)
- Conseil Departemental de la Marne (France)
- Grand Reims (France)
The liquid-liquid extraction in-situ product recovery process using oleyl alcohol as extractant effectively extracts p-coumaric acid from the fermentation broth and impacts strain metabolism. Biphasic fermentations increase p-CA production rates and decrease its decarboxylation into 4-vinylphenol.
p-coumaric acid (p-CA) can be produced from D-glucose by an engineered S. cerevisiae strain. p-CA has antimicrobial properties and retro-inhibition activity. Moreover, p-CA is a hydrophobic compound, limiting its accumulation in fermentation broth. To overcome these issues all at once, a liquid-liquid extraction in-situ product recovery process using oleyl alcohol as extractant has been implemented in order to continuously extract p-CA from the broth. Media and pH impacts on strain metabolism were assessed, highlighting p-CA decarboxylase endogenous activity. Biphasic fermentations allowed an increase in p-CA respiratory production rates at both pH assessed (13.65 and 9.45 mg L-1.h-1 at pH 6 and 4.5, respectively) compared to control ones (10.5 and 7.5 mg L-1.h-1 at pH 6 and 4.5, respectively). Biphasic fermentation effects on p-CA decarboxylation were studied showing that continuous removal of p-CA decreased its decarboxylation into 4-vinylphenol at pH 4.5 (57 mg L-1 in biphasic fermentation vs 173 mg L-1 in control one).
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