Influence mechanism of filling ratio on solid-phase denitrification with polycaprolactone as biofilm carrier

In this study, three up-flow fixed-bed bioreactors were constructed with three different filling ratios (filling volume/effective volume: 30%, 60% and 90%) of polycaprolactone (PCL). Above 98% of nitrate removal efficiency was achieved with low accumulations of nitrite and ammonium for each filling ratio. Low filling ratio of PCL had extensive folds and pores that favored the attachment and growth of microorganisms; however, excessive biomass restrained nitrate specific reduction rate (NaSRR). The most dominant genera were Comamonas (0.80-57.64%), Stenotrophomonas (2.59-54.39%), Acidovorax (7.32-23.55%), Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium (0.30-19.74%) and Thermomonas (0.12-14.58%). Nitrate reductase (EC 1.7.99.4), nitrite reductase (EC 1.7.2.1) and nitric oxide reductase (EC 1.7.2.5) predicted by PICRUSt2 were abundant in high influent nitrate load (NaL). According to the analysis of carbon balance model, the utilization rate (eta) of PCL showed a highly positive correlation with influent NaL, indicating reducing filling ratio or HRT might be an effective measure to save cost for nitrate removal.

Automated summary

This study constructed three up-flow fixed-bed bioreactors with different filling ratios of polycaprolactone (PCL) and found that a low filling ratio favored microbial attachment and growth, while a high influent nitrate load promoted the presence of nitrate reductase and nitrite reductase. Therefore, reducing the filling ratio or increasing the hydraulic retention time (HRT) may be effective measures to save costs for nitrate removal.