The fate of antibiotic resistance genes and their influential factors during excess sludge composting in a full-scale plant

The alteration of antibiotic resistance genes (ARGs) during sludge composting has been less studied in a full-scale plant, causing the miss of practical implications for understanding/managing ARGs. Therefore, this study tracked the changes of ARGs and microbial communities in a full-scale plant engaged in excess sludge composting and then explored the key factors regulating ARGs through a series of analyses. After composting, the absolute and relative abundance of ARGs decreased by 91.90% and 66.57%, respectively. Additionally, pathway analysis showed that MGEs, composting physicochemical properties were the most vital factors directly influencing ARGs. Finally, network analysis indicated that Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were the main hosts of ARGs. Based on these findings, it can be known that full-scale composting could reduce ARGs risk to an extent.

Automated summary

By tracking the changes of ARGs and microbial communities in a full-scale plant engaged in excess sludge composting, this study found that full-scale composting could significantly reduce the absolute and relative abundance of ARGs. Pathway analysis showed that MGEs and composting physicochemical properties were the key factors directly influencing ARGs, while network analysis indicated Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria as the main hosts of ARGs in composting.