Xylitol production from plant biomass by Aspergillus niger through metabolic engineering

Xylitol is widely used in the food and pharmaceutical industries as a valuable commodity product. Biotechnological production of xylitol from lignocellulosic biomass by microorganisms is a promising alternative option to chemical synthesis or bioconversion from D-xylose. In this study, four metabolic mutants of Aspergillus niger were constructed and evaluated for xylitol accumulation from D-xylose and lignocellulosic biomass. All mutants had strongly increased xylitol production from pure D-xylose, beechwood xylan, wheat bran and cotton seed hulls compared to the reference strain, but not from several other feed stocks. The triple mutant Delta ladA Delta xdhA Delta sdhA showed the best performance in xylitol production from wheat bran and cotton seed hulls. This study demonstrated the large potential of A. niger for xylitol production directly from lignocellulosic biomass by metabolic engineering.

Automated summary

Xylitol is widely used in the food and pharmaceutical industries, and biotechnological production using microorganisms is a promising alternative to chemical synthesis. This study demonstrated the potential of Aspergillus niger for xylitol production from lignocellulosic biomass through metabolic engineering, with a triple mutant showing the best performance in certain feed stocks.