The effect of cell disruption on the extraction of oil and protein from concentrated microalgae slurries

Novel cell-disruption combinations (autolytic incubation and hypotonic osmotic shock combined with HPH or pH12) were used to investigate the fundamental mass transfer of lipids and proteins from Nannochloropsis slurries (140 mg biomass/g slurry). Since neutral lipids exist as cytosolic globules, their mass transfer was directly dependent on disintegration of cell walls. Complete recovery was obtained with complete physical disruption. HPH combinations exerted more physical disruption and led to higher yields than pH12. In contrast, proteins exist as both cytosolic water-soluble fractions and cell-wall/membrane structural fractions and have a complex extraction behaviour. Mass transfer of cytosolic proteins was dependent on cell-wall disintegration, while that of structural proteins was governed by cell-wall disintegration and severance of protein linkage from the wall/membrane. HPH combinations exerted only physical disruption and were limited to releasing soluble proteins. pH12 combinations hydrolysed chemical linkages in addition to exerting physical disruption, releasing both soluble and structural proteins.

Automated summary

Novel cell-disruption combinations were used to study the mass transfer of lipids and proteins in Nannochloropsis slurries. The transfer of neutral lipids depended on the disintegration of cell walls, while the transfer of proteins was influenced by both cell-wall disintegration and protein linkage severance. Combinations that primarily involved physical disruption released soluble proteins, while combinations that hydrolysed chemical linkages in addition to physical disruption released both soluble and structural proteins.