期刊
BIORESOURCE TECHNOLOGY
卷 345, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.126414
关键词
Laccase; Rhamnolipid; Simultaneous saccharification and; fermentation; Corn stover
资金
- China National Key RD Program [2020YFA0908400, 2019YFA090500, 2021YFC2100100]
- Science and Technology Innovation Program of Hubei Province [2020BBA056]
- Wuhan Science and Technology Plan [2019020701011496]
It was found that rhamnolipid is an effective surfactant that significantly enhances ethanol production by engineered Saccharomyces cerevisiae in the fermentation of corn stover. The combination strategy of laccase pretreatment and rhamnolipid addition further boosts ethanol production.
The effects of laccase pretreatment and surfactant addition in the simultaneous saccharification and fermentation (SSF) of corn stover by engineered Saccharomyces cerevisiae were studied. Surfactants Tween-80, tea saponin and rhamnolipid improved ethanol production in SSF, among which the biosurfactant rhamnolipid reached the highest ethanol yield. At the 6 d in SSF, the ethanol content of addition rhamnolipid of laccase pretreatment corn stover (Lac-CS) and Lac-CS reached 0.73 g/L and 0.56 g/L, which was 2.32 folds and 1.54 folds higher than the control of 0.22 g/L, respectively. These findings suggested that the combination of laccase pretreatment and rhamnolipid addition further improve ethanol production. GC-MS, composition of corn stover, protein concentration of supernatant and glucose content studies were executed to explore the mechanism of combination strategy of laccase pretreatment and rhamnolipid addition enhance ethanol production. This study provides guidance for the application of laccase and surfactant in bioethanol production.
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