Links between carbon/nitrogen ratio, synergy and microbial characteristics of long-term semi-continuous anaerobic co-digestion of food waste, cattle manure and corn straw

Links between carbon/nitrogen (C/N) ratio, synergy and microbial characteristics of anaerobic co-digestion of food waste (FW), cattle manure (CS) and corn straw (CS) were investigated. Digesters with 100% CS, 25% FW + 75% CS, 25% CM + 75% CS suffered acid inhibition, in close association with unbalanced C/N and the resulting recessions of Syntrophomonadaceae and Methanosaeta. Co-digestion overcame C/N imbalance and achieved multiple synergies. Process performance had a positive correlation with Syntrophomonadaceae. Digester with 75% FW + 25% CS had most Syntrophomonadaceae (26.7%) and methane yield (467.3-507.6 mL/g VS) among co digestion trials. Synergy was greater under higher load and exhibited a good correlation with C/N ratio. Co digestion of FW, CM and CS (2:2:1) with suitable C/N ratio (20.79) obtained the greatest synergistic rate (14.6%). Unstable systems were improved by adjusting C/N ratio to 30 via urea, which stimulated Methanosarcina growth therefore enhanced methanogenic pathway diversity and ensured powerful methanogenic functions.

Automated summary

The study investigated the links between C/N ratio, synergy, and microbial characteristics of anaerobic co-digestion of different organic wastes. Co-digestion demonstrated the ability to overcome C/N imbalance, achieve multiple synergies, and improve methane production. Adjusting the C/N ratio of the system led to enhanced methanogenic functions and system stability.