Microwave hydrothermal processing of Undaria pinnatifida for bioactive peptides

Microwave hydrothermal processing was employed to obtain valuable gelling or bioactive fractions from U. pinnatifida, assessing the processing conditions following a biorefinery concept. It was identified a relevant impact on the antioxidant properties, sulfate, protein and oligosaccharides content, with the highest values above 200 degrees C, although the maximum in fucose was obtained at 160 degrees C. The lowest temperature involved the highest minerals and sulfate content of the solid phases. Rheology indicated that hydrothermal treatment at 160 degrees C is adequate to extract alginates with structural and viscoelastic properties similar to those commercially available. The incorporation of the hydrothermal residual solids in the proposed alginate matrices favored the development of systems with potential non-food applications applications. Selected extracts, after an intensification stage using ultrasound, featured interesting biological activities for two human cancer cell lines (A2780; HeLa 229) with percentage of cellular inhibition > 83 and 57%, without positive effects on A549 and HCT-116.

Automated summary

Microwave hydrothermal processing was used to extract valuable components from U. pinnatifida, with different processing conditions impacting antioxidant properties and content of minerals, proteins, and oligosaccharides. The most suitable temperature for extracting alginates was found to be 160 degrees C, showing potential for non-food applications after incorporation into alginate matrices. Selected extracts exhibited promising biological activities against human cancer cell lines after an intensification stage, with high cellular inhibition percentages observed.