期刊
BIORESOURCE TECHNOLOGY
卷 344, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.126280
关键词
Aeromonas media SZW3; Chloramphenicol (CAP) biodegradation; Biodegradation pathway; Genome analysis
资金
- Key-Area Research and Development Program of Guangdong Province [2018B20205003]
- National Natural Science Foundation of China [U1901601, 41703096, 41807143]
- Research & Development Program of Key Field of Guangdong [2019B020218002]
- Natural Science Foundation of Guangdong Province [2020A1515011222]
- Science and Technology Innovation and Development Special Fund Foundation and Application Basic Research Project [202102020264]
- Key-Area Research and Development Grogram of Guangdong Province [2018B02026001]
- National Science & Technology Fundamental Resources Investigation Program of China [2018FY100300]
- GDAS' Project of Science and Technology Development [2020GDASYL-20200104024]
A bacterium, Aeromonas media SZW3, capable of degrading chloramphenicol (CAP) efficiently was isolated, with the study identifying thirteen possible biodegradation products and proposing three potential biodegradation pathways. Genome analysis suggested specific genes responsible for CAP biodegradation, providing new insights into the fate of CAP in the environment.
The overuse of chloramphenicol (CAP) due to its low price is detrimental to ecological safety and human health. An earthworm gut content dwelling bacterium, Aeromonas media SZW3, was isolated with capability of CAP biodegradation, and the CAP degradation efficiency reached 55.86% at day 1 and 67.28% at day 6. CAP biodegradation kinetics and characteristic of strain SZW3 determined the factors that affect CAP biodegradation. Thirteen possible biodegradation products were identified, including three novel biodegradation products (CP1, CP2 and CP3), and three potential biodegradation pathway were proposed. Biodegradation reactions include amide bond hydrolysis, nitro group reduction, acetylation, aminoacetylation, dechlorination and oxidation. Genome analysis suggested that the coding gene of RarD (CAP resistance permease), CAP O-acetyltransferase, nitroreductase and haloalkane dehalogenase may be responsible for CAP biodegradation. The proposed complete biodegradation pathway and genome analysis by strain SZW3 provide us new insight of the transformation route and fate of CAP in the environment.
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