Enhanced enzymatic saccharification of sorghum straw by effective delignification via combined pretreatment with alkali extraction and deep eutectic solvent soaking

Hydrogen bond donor (HBD) in ChCl-based deep eutectic solvent (DESs) had significant influence on the Sorghum straw (SS) pretreatment. Lactic acid (LAC) was chosen as the appropriate HBD for preparing ChCl-based DES to pretreat Sorghum straw (SS). Furthermore, sequential pretreatment with dilute sodium hydroxide (0.75 wt%) for 1 h at 121 degrees C and ChCl:LAC soaking at 140 degrees C for 40 min was applied to pretreat SS for removing lignin (78.4%) and xylan (67.6%). Hydrolysis for 72 h, the reducing sugar yield reached 94.9%. Moreover, relationships of delignification and xylan removal with saccharification were explored after pretreatment. Finally, the fermentability of SS-hydrolysates was verified by bioethanol fermentation by S. cerevissiae with the yield of 0.45 g ethanol/g glucose. No significant inhibition was observed on ethanol fermentation. Obviously, establishment of high-efficient combination pretreatment with alkali extraction and ChCl:LAC soaking was successfully demonstrated for enhancing enzymatic saccharification of SS.

Automated summary

The study found that the hydrogen bond donor (HBD) in ChCl-based deep eutectic solvent (DES) had a significant impact on the removal of lignin and xylan during the pretreatment of sorghum straw. By sequentially pretreating with dilute sodium hydroxide and soaking in ChCl:LAC, the enzymatic saccharification efficiency of SS was significantly improved.