期刊
BIORESOURCE TECHNOLOGY
卷 341, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125782
关键词
Microbial products; Carotenoids; Corynebacterium; Multi copy integration; Metabolic engineering
资金
- Singapore-MIT Alliancefor Research and Technology (SMART) program entitled Disruptive & Sustainable Technologies for Agricultural Precision (DiSTAP)
This study successfully achieved efficient production of alpha-carotene in Corynebacterium glutamicum using a combined pathway engineering approach, which involved evaluating the performance of different cyclases and analyzing key metabolic intermediates to determine flux bottlenecks in the carotenoid biosynthesis pathway.
The carotenoid, alpha-carotene, is very beneficial for human health and wellness, but microbial production of this compound is notoriously difficult, due to the asymmetric rings on either end of its terpenoid backbone. Here, we report for the first time the efficient production of alpha-carotene in the industrial bacterium Corynebaterium glutamicum by using a combined pathway engineering approach including evaluation of the performance of different cyclases and analysis of key metabolic intermediates to determine flux bottlenecks in the carotenoid biosynthesis pathway. A multi-copy chromosomal integration method was pivotal in achieving stable expression of the cyclases. In fed-batch fermentation, 1,054 mg/L of alpha-carotene was produced by the best strain, which is the highest reported titer achieved in microbial fermentation. The success of increased alpha-carotene production suggests that the multi-copy chromosomal integration method can be a useful metabolic engineering tool for overexpression of key enzymes in C. glutamicum and other bacterium as well.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据