期刊
BIORESOURCE TECHNOLOGY
卷 343, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.126087
关键词
Ultrasound; Switchable solvent; Wet microalgae; Biodiesel
资金
- National Key Research and Development Program -China [2017YFE0122800]
- Zhejiang Provincial Key Research and Development Program-China [2020C04006]
In this study, ultrasound-assisted TEPDA was used to destruct the thick cell wall of Nannochloropsis oceanica, reducing cell wall thickness and achieving efficient lipid extraction.
To facilitate the lipid extraction from Nannochloropsis oceanica with thick cell wall using switchable hydrophilicity solvent, ultrasound-assisted N, N, N', N'-tetraethyl-1,3-propanediamine (TEPDA) was used to effectively destruct the cell wall. TEPDA cations were adsorbed on the cells via electrostatic force and formed the electrondonor-acceptor (EDA) complex with the hydroxyl groups in cellulose. This broke the hydrogen-bonding interactions between cellulose chains and stripped them from cell wall, thus reducing the cell wall thickness from 141 nm to 68.6 nm. Moreover, TEPDA cations neutralized the negatively charged phospholipid bilayers, decreasing the cell surface zeta potential from-27.5 eV to-14.1 eV. The local electrostatic equilibrium led to cell membrane leakage. The ultrasound promoted the stripping of the cellulose chains at a power intensity of 0.5 W/mL and frequency of 20 kHz, achieving the lipid extraction efficiency of 98.2% within 2 h at a volume ratio of 1:4 of wet microalgae to TEPDA.
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