Development of bioactive solid support for immobilized Lactobacillus casei biofilms and the production of lactic acid

Polypropylene was modified to contain chitosan and evaluate its ability to generate Lactobacillus casei biofilms and their lactic acid production. Biofilm formation was carried out in either rich or minimal media. The chitosan-modified polypropylene harbored similar to 37% more cells than the control polypropylene. The biofilms from the chitosan-modified polypropylene grown in rich medium produced similar to 2 times more lactic acid after 72 h of incubation than the control suspended cells. There was no significant difference in the production of lactic acid after 72 h by L. casei biofilms on the chitosan-modified polypropylene grown in minimal media as compared with cells in suspension after 48 h and 72 h of incubation. Infrared spectroscopy confirmed higher deposition of nutrients and biomass on the chitosan-modified polypropylene as compared to the chitosan-free polypropylene. Electron and atomic force microscopy confirmed thicker biofilms when rich media were used to grow them as compared to minimal medium.

Automated summary

The modification of polypropylene with chitosan was found to enhance the generation of Lactobacillus casei biofilms and their lactic acid production. Biofilms grown in rich media produced significantly more lactic acid, while the chitosan-modified polypropylene harbored more cells compared to the control. The deposition of nutrients and biomass on the chitosan-modified polypropylene was confirmed to be higher, with thicker biofilms observed when grown in rich media.