期刊
BIOORGANIC CHEMISTRY
卷 116, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bioorg.2021.105357
关键词
Gynostemma pentaphyllum; Triterpenoid saponin; Minor gypenosides; Sirt1
资金
- National Key Research and Development Project [2019YFC1711000]
- Scientific Research Innovation Program of Jiangsu Graduate [KYCX20_0676]
- Qing Lan Project of Jiangsu Higher Education Institutions
- Funding of Double First-rate Discipline Innovation Team [CPU2018GF05, CPU2018GY31]
This study isolated and structurally characterized novel, minor gypenosides from Gynostemma pentaphyllum, and evaluated their Sirt1 agonist activity. Results showed satisfactory Sirt1 agonist activity in four saponins, indicating the potential for further research on G. pentaphyllum as a source of Sirt1 agonists.
Gynostemma pentaphyllum (Thunb.) Makino (Cucurbitaceae family) is a perennial creeping plant with a common Chinese name of south ginseng. To date, more than 250 individual saponins with dammarane-type skeleton have been isolated from G. pentaphyllum. The purpose of this study was the isolation and structural characterization of novel, minor gypenosides from G. pentaphyllum and evaluation of their Sirt1 agonist activity. Individual saponins from G. pentaphyllum were isolated and purified by a variety of chromatography techniques, and their structures were elucidated by means of various spectroscopic analysis and comparision with the reported data. Sirt1 enzyme activity detection kit was used to preliminarily evaluate the Sirt1 agonist activity of thirty three individual saponins purified from G. pentaphyllum. Fourteen new triterpenoid saponins named gypenoside CIICXV (1-14) along with twenty six known compounds (15-40) were isolated from G. pentaphyllum. Thirty three of all the isolates were screened for Sirt1 agonist activity, and the results showed that three dammaranetype saponins (2, 18, 37) and one cucurbitane-type saponin 33 exhibited satisfactory Sirt1 agonist activity. These findings suggested that G. pentaphyllum was worthy of further investigation to find small molecule Sirt1 agonist and facilitate their utilization as south ginseng.
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