期刊
BIOMEDICAL CHROMATOGRAPHY
卷 36, 期 3, 页码 -出版社
WILEY
DOI: 10.1002/bmc.5273
关键词
determination; LC-MS; MS; metabolite; tenofovir; tenofovir alafenamide
类别
资金
- Ability of Clinical Trial Organization in Suzhou Science and Technology Town Hospital Phase I Clinical Research Department [SLT2021002]
A simple and fast liquid chromatography-tandem mass spectrometry method was established and validated for the simultaneous determination of tenofovir alafenamide (TAF) and tenofovir (TNF) in human plasma, with acceptable accuracy and precision in the concentration ranges tested.
A simple and fast liquid chromatography-tandem mass spectrometry method was established and validated for the simultaneous determination of tenofovir alafenamide (TAF) and tenofovir (TNF) in human plasma. A simple protein precipitation procedure was employed to extract analytes from plasma. Chromatographic separation was performed on an Eclipse Plus C-18 column utilizing a fast gradient elution starting with 2% of 2 mM ammonium acetate-formic acid (100/0.1, v/v) followed by increasing the percentage of acetonitrile. Detection was performed on a tandem mass spectrometer equipped with an electrospray ionization source operated in the positive ionization mode, using the transitions m/z 477.2 -> m/z 346.1 for TAF and m/z 288.1 -> m/z 176.1 for TNF. TAF-d(5) and TNF-d(7) were used as the internal standard of TAF and TNF, respectively. The method was validated in the concentration ranges 1.25-500 ng/mlfor TAF and 0.300-15.0 ng/ml for TNF with acceptable accuracy and precision.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据