期刊
BIOMEDICAL CHROMATOGRAPHY
卷 36, 期 3, 页码 -出版社
WILEY
DOI: 10.1002/bmc.5288
关键词
amlexanox; bioavailability; LC-MS; MS method; pharmacokinetics
类别
资金
- Fundamental Research Funds for the Central Universities [202013021]
A sensitive LC-MS/MS method for the determination of amlexanox in rat plasma was successfully established and validated, and applied to pharmacokinetic study. The method involved the use of Capcell Pak C18 column and triple quadrupole mass in MRM for detection, with calibration curves ranging from 50 to 2000 ng/mL.
Amlexanox, an anti-inflammatory and anti-allergic agent, has been widely used clinically for the treatment of canker sores, asthma, and allergic rhinitis. Recently, amlexanox has received considerable attention in curing nonalcoholic fatty liver diseases and hepatitis virus infection. Herein, we first established a sensitive high-performance liquid chromatography-tandem mass spectrum (LC-MS/MS) method for the determination of amlexanox in rat plasma. Propranolol was used as the internal standard (IS). Using a simple protein precipitation method, the amlexanox and IS were separated with Capcell Pak C18 column (2.0 x 50 mm, 5 mu m) and eluted with water and acetonitrile each containing 0.1% formic acid using gradient elution condition at a flow rate of 0.4 mL center dot min(-1). Amlexanox and IS were detected by a triple quadrupole mass in multiple reactive monitoring (MRM) under the transitions of m/z 299.2 -> 281.2 and m/z 259.9 -> 116.1 with positive electrospray ionization, respectively. The calibration curves of amlexanox were established with the range of 50 to 2000 ng center dot mL(-1) (r(2) > 0.99). The validation method consisted of selectivity, accuracy, precision, carryover effect, matrix effect, recovery, dilution effect, and stability. The fully validated method was successfully applied to the pharmacokinetic study of amlexanox in Wistar rats.
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