A poly(ethylene glycol) three-dimensional bone marrow hydrogel

Three-dimensional (3D) hydrogels made from synthetic polymers have emerged as in vitro cell culture platforms capable of representing the extracellular geometry, modulus, and water content of tissues in a tunable fashion. Hydrogels made from these otherwise non-bioactive polymers can be decorated with short peptides derived from proteins naturally found in tissues to support cell viability and direct phenotype. We identified two key limitations that limit the ability of this class of materials to recapitulate real tissue. First, these environments typically display between 1 and 3 bioactive peptides, which vastly underrepresents the diversity of proteins found in the extracellular matrix (ECM) of real tissues. Second, peptides chosen are ubiquitous in ECM and not derived from proteins found in specific tissues, per se. To overcome this critical limitation in hydrogel design and functionality, we developed an approach to incorporate the complex and specific protein signature of bone marrow into a poly (ethylene glycol) (PEG) hydrogel. This bone marrow hydrogel mimics the elasticity of marrow and has 20 bone marrow-specific and cell-instructive peptides. We propose this tissue-centric approach as the next generation of 3D hydrogel design for applications in tissue engineering and beyond.

Automated summary

Three-dimensional hydrogels made from synthetic polymers can mimic the extracellular matrix (ECM) of real tissues, but they have limitations in terms of the diversity and specificity of bioactive peptides. To overcome these limitations, researchers have developed a bone marrow hydrogel that incorporates the complex and specific protein signature of bone marrow. This tissue-centric approach allows for better replication of the elasticity and structure of bone marrow, and provides a wide range of bone marrow-specific and cell-instructive peptides.