Influence of Divalent Metal Ions on the Precipitation of the Plasma Protein Fibrinogen

Fibrinogen nanofibers are very attractive biomaterials to mimic the native blood dot architecture. Previously, we reported the self-assembly of fibrinogen nanofibers in the presence of monovalent salts and have now studied how divalent salts influence fibrinogen precipitation. Although the secondary fibrinogen structure was significantly altered with divalent metal ions, morphological analysis revealed exclusively smooth fibrinogen precipitates. In situ monitoring of the surface roughness facilitated predicting the tendency of various salts to form fibrinogen fibers or smooth films. Analysis of the chemical composition revealed that divalent salts were removed from smooth fibrinogen films upon rinsing while monovalent Na+ species were still present in fibrinogen fibers. Therefore, we assume that the decisive factor controlling the morphology of fibrinogen precipitates is direct ion-protein contact, which requires disruption of the ion-surrounding hydration shells. We conclude that in fibrinogen aggregates, this mechanism is effective only for monovalent ions, whereas divalent ions are limited to indirect fibrinogen adsorption.

Automated summary

The interaction between divalent salts and fibrinogen affects the morphology of fibrinogen precipitates, with divalent ions being removed from smooth fibrinogen films while monovalent species are present in fibrinogen fibers. The study suggests that direct ion-protein contact is the decisive factor controlling the morphology of fibrinogen precipitates, with monovalent ions having a more direct impact compared to divalent ions.