期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 200, 期 7, 页码 3176-3186出版社
SPRINGERNATURE
DOI: 10.1007/s12011-021-02909-6
关键词
Co; BGSr2% nanocomposite; Bone regeneration; Osteocalcin
In this study, different combinations of materials and cells were used to treat full-thickness bone defects in rabbits, with the Co/BGSr2% + MSC group showing the best treatment outcomes. Through X-ray radiography, histology, and immunohistochemistry, it was found that the Co/BGSr2% nanocomposite scaffold seeded with MSCs can accelerate bone regeneration, with osteocalcin protein playing a key role in this process.
Bone self-healing is limited and requires additional or external intervention to promote and accelerate bone regeneration. Therefore, the aim of this study was to investigate the potential capacity of hydrogel collagen (Co) nanocomposite alone, and in combination with 2% strontium (Co/BGSr2%) in presence of mesenchymal stem cells (MSCs) in full-thickness bone defect regeneration in the rabbit animal model. A total of 72 New Zealand white rabbits were randomly divided in 6 groups of 12 rabbits with full-thickness bone defect. In five groups, the bone defect was treated with MSC, Co, Co/BGSr2%, Co + MSCs, and Co/BGSr2% + MSCs. No treatment was done in the control group. The treatments were assessed radiographically, histopathologically, and immunohistochemically on days 14, 28, 42, and 56 post-treatment. The highest radiographical and histological scores were belonged to the Co/BGSr2% + MSC followed by Co + MSCs, Co/BGSr2%, Co, MSC, and the control groups. The highest and lowest mean expression level of osteocalcin was detected in the Co/BGSr2% + MSC and control groups by 28th dayof post-implantation, respectively. In contrast, the highest and lowest mean expression level of osteocalcin on day 56 post-implantation was belonged to the control and Co/BGSr2% + MSC, respectively. The Co/BGSr2% nanocomposite scaffold seeded with MSC can accelerate bone regeneration resulted from osteoblastic production of osteocalcin protein. Therefore, collagen hydrogel combined with 2% strontium in nanocomposite form is a suitable candidate scaffold for bone tissue engineering.
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