LLPSDB v2.0: an updated database of proteins undergoing liquid-liquid phase separation in vitro

Emerging evidences have suggested that liquid-liquid phase separation (LLPS) of proteins plays a vital role both in a wide range of biological processes and in related diseases. Whether a protein undergoes phase separation not only is determined by the chemical and physical properties of biomolecule themselves, but also is regulated by environmental conditions such as temperature, ionic strength, pH, as well as volume excluded by other macromolecules. A web accessible database LLPSDB was developed recently by our group, in which all the proteins involved in LLPS in vitro as well as corresponding experimental conditions were curated comprehensively from published literatures. With the rapid increase of investigations in biomolecular LLPS and growing popularity of LLPSDB, we updated the database, and developed a new version LLPSDB v2.0. In comparison of the previously released version, more than double contents of data are curated, and a new class `Ambiguous system' is added. In addition, the web interface is improved, such as that users can search the database by selecting option `phase separation status' alone or combined with other options. We anticipate that this updated database will serve as a more comprehensive and helpful resource for users. Availability and implementation: LLPSDB v2.0 is freely available at: http://bio-comp.org.cn/llpsdbv2. Contact: zhuqingzhang@ucas.ac.cn Supplementary information: Supplementary data are available at Bioinformatics online.

Automated summary

Emerging evidence suggests that liquid-liquid phase separation (LLPS) of proteins has a crucial role in various biological processes and related diseases. A database called LLPSDB was developed to gather comprehensive data on proteins involved in LLPS and their experimental conditions. The updated version, LLPSDB v2.0, contains more than double the amount of curated data and improved web interface, providing a more comprehensive and helpful resource for users.