TDFragMapper: a visualization tool for evaluating experimental parameters in top-down proteomics

Motivation: We present a new software-tool allowing an easy visualization of fragment ions and thus a rapid evaluation of key experimental parameters on the sequence coverage obtained for the MS/MS (tandem mass spectrometry) analysis of intact proteins. Our tool can process data obtained from various deconvolution and fragment assignment software. Results: We demonstrate that TDFragMapper can rapidly highlight the experimental fragmentation parameters that are critical to the characterization of intact proteins of various size using top-down proteomics.

Automated summary

This new software tool allows easy visualization of fragment ions and rapid evaluation of key experimental parameters for the analysis of intact proteins. It can process data obtained from different software and is useful for top-down proteomics.