期刊
BIOINFORMATICS
卷 38, 期 4, 页码 954-961出版社
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btab760
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资金
- Swedish National Research Council [VR-NT-2016-03798]
- Swedish Research Council
The study introduces a fold-and-dock method utilizing deep learning for predicting protein-protein contact distances, which can simultaneously predict the tertiary and quaternary structure of a protein pair, outperforming traditional methods in terms of docking success rates.
Motivation: In the last decade, de novo protein structure prediction accuracy for individual proteins has improved significantly by utilising deep learning (DL) methods for harvesting the co-evolution information from large multiple sequence alignments (MSAs). The same approach can, in principle, also be used to extract information about evolutionary-based contacts across protein-protein interfaces. However, most earlier studies have not used the latest DL methods for inter-chain contact distance prediction. This article introduces a fold-and-dock method based on predicted residue-residue distances with trRosetta. Results: The method can simultaneously predict the tertiary and quaternary structure of a protein pair, even when the structures of the monomers are not known. The straightforward application of this method to a standard dataset for protein-protein docking yielded limited success. However, using alternative methods for generating MSAs allowed us to dock accurately significantly more proteins. We also introduced a novel scoring function, PconsDock, that accurately separates 98% of correctly and incorrectly folded and docked proteins. The average performance of the method is comparable to the use of traditional, template-based or ab initio shape-complementarity-only docking methods. Moreover, the results of conventional and fold-and-dock approaches are complementary, and thus a combined docking pipeline could increase overall docking success significantly. This methodology contributed to the best model for one of the CASP14 oligomeric targets, H1065.
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