期刊
BIOELECTROCHEMISTRY
卷 143, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2021.107982
关键词
Carbon nanotubes; Field-effect transistor; Biosensor; Electrical immunosensor; Severe acute respiratory syndrome coronavirus-2
资金
- King Abdulaziz City for Science and Technology (General Directorate for Research and Innovation Support (GDRIS)) at King Abdulaziz University, Jeddah, Saudi Arabia
- fast-track program for COVID-19 research [5-20-01-009-0074]
A fast, easy-to-use, low-cost, and quantitative electrochemical biosensor based on CNT-FET has been developed for quick and accurate detection of SARS-CoV-2 S1 antigen in fortified saliva samples. The biosensor exhibits high sensitivity, selectivity, and can save time, making it a valuable platform for rapid detection of SARS-CoV-2 S1 antigen from patient saliva.
The large-scale diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important for traceability and treatment during pandemic outbreaks. We developed a fast (2-3 min), easy-to-use, low-cost, and quantitative electrochemical biosensor based on carbon nanotube field-effect transistor (CNT-FET) that allows digital detection of the SARS-CoV-2 S1 in fortifited saliva samples for quick and accurate detection of SARS-CoV-2 S1 antigens. The biosensor was developed on a Si/SiO2 surface by CNT printing with the immobilization of a anti-SARS-CoV-2 S1. SARS-CoV-2 S1 antibody was immobilized on the CNT surface between the S-D channel area using a linker 1-pyrenebutanoic acid succinimidyl ester (PBASE) through non-covalent interaction. A commercial SARS-CoV-2 S1 antigen was used to characterize the electrical output of the CNT-FET biosensor. The SARS-CoV-2 S1 antigen in the 10 mM AA buffer pH 6.0 was effectively detected by the CNT-FET biosensor at concentrations from 0.1 fg/mL to 5.0 pg/mL. The limit of detection (LOD) of the developed CNT-FET biosensor was 4.12 fg/mL. The selectivity test was per-formed by using target SARS-CoV-2 S1 and non-target SARS-CoV-1 S1 and MERS-CoV S1 antigens in the 10 mM AA buffer pH 6.0. The biosensor showed high selectivity (no response to SARS-CoV-1 S1 or MERS-CoV S1 antigen) with SARS-CoV-2 S1 antigen detection in the 10 mM AA buffer pH 6.0. The biosensor is highly sensitive, saves time, and could be a helpful platform for rapid detection of SARS-CoV-2 S1 antigen from the patients saliva. (C) 2021 The Authors. Published by Elsevier B.V.
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