期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1863, 期 12, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbamem.2021.183757
关键词
GLUT1; TXNIP (thioredoxin-interacting protein); Glucose metabolism; Nanodiscs; Electron microscopy; PI(4,5)P-2
资金
- National Institutes of Health [R01-GM120129]
The interaction between GLUT1 and TXNIP in regulating cellular glucose uptake is essential and requires phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2 or PIP2).
GLUT1 is a major glucose facilitator expressed ubiquitously among tissues. Upregulation of its expression plays an important role in the development of many types of cancer and metabolic diseases. Thioredoxin-interacting protein (TXNIP) is an a-arrestin that acts as an adaptor for GLUT1 in clathrin-mediated endocytosis. It regulates cellular glucose uptake in response to both intracellular and extracellular signals via its control on GLUT1-4. In order to understand the interaction between GLUT1 and TXNIP, we generated GLUT1 lipid nanodiscs and carried out isothermal titration calorimetry and single-particle electron microscopy experiments. We found that GLUT1 lipid nanodiscs and TXNIP interact in a 1:1 ratio and that this interaction requires phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2 or PIP2).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据