Surfactin cyclic lipopeptides change the plasma membrane composition and lateral organization in mammalian cells

The specific structure and composition of the cell plasma membrane (PM) is crucial for many cellular processes and can be targeted by various substances with potential medical applications. In this context, biosurfactants (BS) constitute a promising group of natural compounds that possess several biological functions, including anticancer activity. Despite the efficiency of BS, their mode of action had never been elucidated before. Here, we demonstrate the influence of cyclic lipopeptide surfactin (SU) on the PM of CHO-K1 cells. Both FLIM and svFCS experiments show that even a low concentration of SU causes significant changes in the membrane fluidity and dynamic molecular organization. Further, we demonstrate that SU causes a relevant dose-dependent reduction of cellular cholesterol by extracting it from the PM. Finally, we show that CHO-25RA cells characterized by increased cholesterol levels are more sensitive to SU treatment than CHO-K1 cells. We propose that sterols organizing the PM raft nanodomains, constitute a potential target for SU and other biosurfactants. In our opinion, the anticancer activity of biosurfactants is directly related with the higher cholesterol content found in many cancer cells.

Automated summary

The study demonstrates that cyclic lipopeptide surfactin (SU) significantly influences the plasma membrane of CHO-K1 cells, reducing cellular cholesterol levels by extracting it from the membrane. CHO-25RA cells with increased cholesterol content are more sensitive to SU treatment. The research suggests that cholesterol in membrane raft nanodomains is a potential target for SU and other biosurfactants, and that the anticancer activity of biosurfactants may be linked to higher cholesterol levels in cancer cells.