期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1863, 期 1, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbabio.2021.148503
关键词
CO2 concentrating mechanism; CCM; NDH-1; Inorganic carbon; Rubisco; Photosynthesis
资金
- United States Department of Energy, Office of Basic Energy Sciences [DE-FG02-08ER15968]
- U.S. Department of Energy (DOE) [DE-FG02-08ER15968] Funding Source: U.S. Department of Energy (DOE)
The study focused on investigating the role of conserved histidine and cysteine residues in the CupB protein in cyanobacteria using a Synechococcus PCC7942 expression system. Results showed that the most severe defects in activity among the targeted residues were due to a substitution of CupB-His86, and proteomic analysis revealed that certain amino acid substitution mutations of CupB induced bicarbonate uptake proteins to a greater extent.
The uptake of inorganic carbon in cyanobacteria is facilitated by an energetically intensive CO2-concentrating mechanism (CCM). This includes specialized Type-1 NDH complexes that function to couple photosynthetic redox energy to CO2 hydration forming the bicarbonate that accumulates to high cytoplasmic concentrations during the operation of the CCM, required for effective carbon fixation. Here we used a Synechococcus PCC7942 expression system to investigate the role of conserved histidine and cysteine residues in the CupB (also designated, ChpX) protein, which has been hypothesized to participate in a vectoral CO2 hydration reaction near the interface between CupB protein and the proton-pumping subunits of the NDH-1 complex. A homology model has been constructed and most of the targeted conserved residues are in the vicinity of a Zn ion modeled to form the catalytic site of deprotonation and CO2 hydration. Growth and CO2 uptake assays show that the most severe defects in activity among the targeted residues are due to a substitution of the predicted Zn ligand, CupB-His86. Mutations at other sites produced intermediate effects. Proteomic analysis revealed that some amino acid substitution mutations of CupB caused the induction of bicarbonate uptake proteins to a greater extent than complete deletion of CupB, despite growth under CO2-enriched conditions. The results are discussed in terms of hypotheses on the catalytic function of this unusual enzyme.
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