期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 576, 期 -, 页码 73-79出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2021.08.078
关键词
microRNA-199b-3p; Phospholipase C epsilon; Prostate cancer; Malignant proliferation
资金
- National Natural Science Foundation of China (NSFC) [81802543]
miR-199b-3p expression is significantly lower in PCa compared to benign prostatic hyperplasia, and is associated with poor prognosis. It can inhibit the malignant proliferation of PCa cells by down-regulating PLC epsilon.
Objectives: MicroRNA-199b-3p (miR-199b-3p) plays a crucial role in the malignant development of various cancers, but little known in prostate cancer (PCa). The aim of our study was to demonstrate the function of miR-199b-3p in PCa. Methods: Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect miR-199b-3p expression in PCa and benign prostatic hyperplasia (BPH) tissue samples. In addition, we examined the relationship between the poor prognosis in PCa and miR-199b-3p. Western blot was used to analyze the expression of Phospholipase C epsilon (PLC epsilon). CCK8 and colony-forming assays were applied to detect the proliferation of PCa. EdU assay is used to detect PCa cells uptake of EdU. Luciferase reporter assay was applied to analyze the binding between miR-199b-3p and PLC epsilon. Results: It has been shown that miR-199b-3p in PCa was significantly lower than that in benign prostatic hyperplasia and correlated with poor prognosis. Meanwhile, upregulation of miR-199b-3p can prominently inhibit the proliferation of PCa cells, while its down-regulation triggered opposite result. PLC epsilon was identified as the downstream binding target gene and negatively associated with that of miR-199b-3p. Conclusion: miR-199b-3p suppresses malignant proliferation by inhibiting PLC epsilon in prostate cancer in vitro and vivo. (C) 2021 Published by Elsevier Inc.
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