Aphid salivary protein Mp1 facilitates infestation by binding phloem protein 2-A1 in Arabidopsis

We have previously demonstrated that Arabidopsis (Arabidopsis thaliana) phloem protein PP2-A1 is an integral component of resistance to the green peach aphid (Myzus persicae). Here, we report that M. persicae overcomes the resistance of PP2-A1 by using the salivary protein Mp1 as an energetic effector and an interactor of AtPP2-A1. Using the RNA interference technique, we demonstrated that Mp1 plays an essential role in the phloem-feeding activity of M. persicae. When the Mp1 gene was silenced, aphids incurred serious impairments not only in phloem-feeding activity, but also in survival and fertility. In essence, phloem-feeding activity was attributed to the molecular interaction between Mp1 and AtPP2A1. The Mp1 and AtPP2-A1 interactions were localized to plant cell membranes by coimmunoprecipitation and bimolecular fluorescence complementation experiments. Furthermore, the interaction was found to be required for aphid feeding on Arabidopsis phloem. Overall, our results suggest that Mp1 is an important effector of M. persicae and interacts with AtPP2-A1 to facilitate infestation in the plant tissue by this insect. (c) 2021 Published by Elsevier Inc.

Automated summary

The green peach aphid overcomes the resistance of PP2-A1 in Arabidopsis by using the salivary protein Mp1 as an energetic effector, playing an essential role in phloem-feeding activity. The molecular interaction between Mp1 and AtPP2-A1 is crucial for aphid infestation in plant tissues.