期刊
ARCHIVES OF VIROLOGY
卷 167, 期 3, 页码 995-998出版社
SPRINGER WIEN
DOI: 10.1007/s00705-022-05389-8
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- Beijing Municipal Education Committee [CITTCD201904055]
The complete genome sequence of peony leafroll-associated virus (PLRaV) was determined and its genetic characteristics were analyzed. The study found that PLRaV shares similarities with other members of the genus Ampelovirus in terms of genome structure and sequence. Phylogenetic analysis showed that PLRaV belongs to subgroup I of Ampelovirus.
The complete genome sequence of peony leafroll-associated virus (PLRaV) was determined by deep sequencing of ribosomal-RNA-depleted total RNA extracted from a peony plant exhibiting leafroll symptoms. Further PCR and RACE analysis showed that the PLRaV genome consists of 15,406 nucleotides and contains 10 putative open reading frames, with an organization typical of members of the genus Ampelovirus, family Closteroviridae. Amino acid sequence comparisons showed that the viral heat shock protein 70 homolog (HSP70h) shared the highest sequence identity (41.7%) with the corresponding region of grapevine leafroll-associated virus 1, and the coat protein (CP) and RNA-dependent RNA polymerase (RdRp) shared the highest sequence identity (32.1% and 52.3%, respectively) with grapevine leafroll-associated virus 13. Phylogenetic analysis of the HSP70h, CP, and RdRp aa sequences showed that PLRaV clustered with members of subgroup I of the genus Ampelovirus.
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